Hotspot

ccsA

SSC · 134780-135751 · 972 bp

Pi 0.46

MarkerSeek score 81.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 396
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1348 bp	0.5	59.4
ccsA_p2	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1347 bp	0.5	59.4
ccsA_p3	GTGAAATCGGTAGACACGCT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1630 bp	0.5	59.0
ccsA_p4	TGAAATCGGTAGACACGCTG	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1629 bp	0.5	59.0
ccsA_p5	ACGGGGAACTCAAAAAGCTT	GGTGTTTGTGAGAACCCCTT	45.0	50.0	57.9	57.9	1870 bp	0.5	58.9