Hotspot

trnL-UAG-ccsA

SSC · 134688-134779 · 92 bp

Back to Inga

Pi 0.432
MarkerSeek score 75.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
92
variable_sites
35
pi
0.432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT AATGCGATTTTAGAGCGGGT 50.0 45.0 58.0 58.0 572 bp 0.5 59.6
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG AATGCGATTTTAGAGCGGGT 50.0 45.0 58.0 58.0 571 bp 0.5 59.6
trnL-UAG-ccsA_p3 GTGAAATCGGTAGACACGCT TGCGATTTTAGAGCGGGTAC 50.0 50.0 58.0 58.1 570 bp 0.5 59.4
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG TGCGATTTTAGAGCGGGTAC 50.0 50.0 58.0 58.1 569 bp 0.5 59.4
trnL-UAG-ccsA_p5 ACGGGGAACTCAAAAAGCTT AATGCGATTTTAGAGCGGGT 45.0 45.0 57.9 58.0 1094 bp 0.5 59.2