Hotspot

rpl32-trnL-UAG

SSC · 134146-134607 · 462 bp

Back to Inga

Pi 0.445
MarkerSeek score 79.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
462
variable_sites
182
pi
0.445
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACGGGGAACTCAAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 542 bp 0.5 59.4
rpl32-trnL-UAG_p2 ACGGGGAACTCAAAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 543 bp 0.5 59.4
rpl32-trnL-UAG_p3 TTAACGACCAAGTAAGCGGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1036 bp 0.5 59.3
rpl32-trnL-UAG_p4 TTAACGACCAAGTAAGCGGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1037 bp 0.5 59.3
rpl32-trnL-UAG_p5 ACGGGGAACTCAAAAAGCTT AATGCGATTTTAGAGCGGGT 45.0 45.0 57.9 58.0 1094 bp 0.5 59.2