Hotspot

psbC-trnS-UGA

LSC · 39645-39886 · 242 bp

Back to Inga

Pi 0.0413
MarkerSeek score 76.30
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
242
variable_sites
10
pi
0.0413
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CAGCGGGATTTGAAAAAGGC GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.3 58.0 402 bp 1.0 79.5
psbC-trnS-UGA_p2 GCAGCGGGATTTGAAAAAGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.3 58.0 403 bp 1.0 79.5
psbC-trnS-UGA_p3 CAGCGGGATTTGAAAAAGGC GCTGAGTGGTTGATAGCTCC 50.0 55.0 58.3 58.1 394 bp 1.0 79.3
psbC-trnS-UGA_p4 CAGCGGGATTTGAAAAAGGC GGCTGAGTGGTTGATAGCTC 50.0 55.0 58.3 58.1 395 bp 1.0 79.3
psbC-trnS-UGA_p5 GCAGCGGGATTTGAAAAAGG GCTGAGTGGTTGATAGCTCC 50.0 55.0 58.3 58.1 395 bp 1.0 79.3