Hotspot

rpoC2

LSC · 27212-31765 · 4554 bp

Back to Indosasa

Pi 0.000596

MarkerSeek score 49.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4554
variable_sites: 9
pi: 0.000596
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GCCAGAGGAAATGCATCTCA	CCGTCATCCCATTTTGAGGA	50.0	50.0	58.0	57.6	301 bp	1.0	76.8
rpoC2_p2	GCCAGAGGAAATGCATCTCA	TCCGTCATCCCATTTTGAGG	50.0	50.0	58.0	57.6	302 bp	1.0	76.8
rpoC2_p3	AGCCAGAGGAAATGCATCTC	CCGTCATCCCATTTTGAGGA	50.0	50.0	57.7	57.6	302 bp	1.0	74.4
rpoC2_p4	AGCCAGAGGAAATGCATCTC	TCCGTCATCCCATTTTGAGG	50.0	50.0	57.7	57.6	303 bp	1.0	74.4
rpoC2_p5	GAGCCAGAGGAAATGCATCT	CCGTCATCCCATTTTGAGGA	50.0	50.0	57.7	57.6	303 bp	1.0	74.4