Hotspot

rbcL

LSC · 56998-58431 · 1434 bp

Back to Indosasa

Pi 0.00291

MarkerSeek score 43.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 10
pi: 0.00291
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTT	50.0	40.0	58.0	56.0	1565 bp	1.0	64.2
rbcL_p2	ATCGAGTAGACCCTGTCGTT	ACTGCATTTCGTAACTGCTT	50.0	40.0	57.9	56.0	1569 bp	1.0	63.3
rbcL_p3	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTTT	50.0	38.1	58.0	56.7	1565 bp	1.0	62.3
rbcL_p4	TAGACCCTGTCGTTGTGAGA	ACTGCATTTCGTAACTGCTT	50.0	40.0	57.7	56.0	1563 bp	1.0	62.0
rbcL_p5	GTAGACCCTGTCGTTGTGAG	ACTGCATTTCGTAACTGCTT	55.0	40.0	57.7	56.0	1564 bp	1.0	61.5