Hotspot

petB

LSC · 73848-75311 · 1464 bp

Back to Indosasa

Pi 0.00166

MarkerSeek score 51.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 9
pi: 0.00166
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1565-1579 bp	1.0	68.1
petB_p2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1565-1579 bp	1.0	67.7
petB_p3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1571-1585 bp	1.0	67.3
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1570-1584 bp	1.0	65.9
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1571-1585 bp	1.0	65.9