Hotspot

psbZ-trnG-UCC

LSC · 12660-13611 · 952 bp

Back to Indosasa

Pi 0.00132
MarkerSeek score 49.40
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
952
variable_sites
5
pi
0.00132
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTGCTTCTCCTGATGGTTGG AGCCTTCCAAGCTAACGATG 50.0 50.0 57.8 58.0 1102-1106 bp 1.0 78.9
psbZ-trnG-UCC_p2 TTGCTTCTCCTGATGGTTGG CCTAGCCTTCCAAGCTAACG 50.0 55.0 57.8 58.1 1105-1109 bp 1.0 78.7
psbZ-trnG-UCC_p3 GCTTCTCCTGATGGTTGGTC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 1100-1104 bp 1.0 78.5
psbZ-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 1103-1107 bp 1.0 78.3
psbZ-trnG-UCC_p5 TTGCTTCTCCTGATGGTTGG CCAACGTCGACTATAACCCC 50.0 55.0 57.8 57.8 1123-1127 bp 1.0 77.4