Hotspot

ndhF

SSC · 105058-107277 · 2220 bp

Back to Indocalamus

Pi 0.00113

MarkerSeek score 35.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 9
pi: 0.00113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAACA	55.0	42.9	57.8	58.3	2363-2365 bp	1.0	68.2
ndhF_p2	GGAGAGATGGGGGAAGAAGA	GGTTTGGTGAATCGGAAACA	55.0	45.0	57.8	56.5	2362-2364 bp	1.0	67.3
ndhF_p3	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAAC	55.0	45.0	57.8	56.5	2363-2365 bp	1.0	67.3
ndhF_p4	AGGAAGGGGAATAAGAGGGG	TGGTTTGGTGAATCGGAAACA	55.0	42.9	57.8	58.3	2344 bp	0.111111	32.0
ndhF_p5	AGGAAGGGGAATAAGAGGGG	GGTTTGGTGAATCGGAAACA	55.0	45.0	57.8	56.5	2343 bp	0.111111	31.1