Hotspot

infA

LSC · 3918-4259 · 342 bp

Back to Indocalamus

Pi 0.00206

MarkerSeek score 31.20

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 342
variable_sites: 3
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TTAAAGCGTATCAGCCGACC	ATACGCACTCTCCTACGTGA	50.0	50.0	58.1	58.0	1051-1070 bp	1.0	81.5
infA_p2	TTAAAGCGTATCAGCCGACC	TCCACTACCAGCACCCTTTA	50.0	50.0	58.1	58.0	1322-1341 bp	1.0	81.0
infA_p3	TTAAAGCGTATCAGCCGACC	TTGCAAACCCACTGTACGAA	50.0	45.0	58.1	58.0	1286-1305 bp	1.0	81.0
infA_p4	TTAAAGCGTATCAGCCGACC	GTAATGCGGCATCTCTTCCA	50.0	50.0	58.1	58.0	1338-1357 bp	1.0	80.9
infA_p5	TTAAAGCGTATCAGCCGACC	TAATGCGGCATCTCTTCCAC	50.0	50.0	58.1	58.0	1337-1356 bp	1.0	80.9