Hotspot

rps16-trnK-UUU

LSC · 78623-79178 · 556 bp

Back to Indocalamus

Pi 0.0035
MarkerSeek score 50.80
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
556
variable_sites
8
pi
0.0035
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 GGTGCTCAACCTACAAGAACT AGTCGCACTTAAAAGCCGAG 47.6 50.0 57.9 58.6 690 bp 1.0 67.3
rps16-trnK-UUU_p2 GGTGCTCAACCTACAAGAACT TAGTCGCACTTAAAAGCCGA 47.6 45.0 57.9 57.3 691 bp 1.0 66.1
rps16-trnK-UUU_p3 GGTGCTCAACCTACAAGAACT TCGCACTTAAAAGCCGAGTA 47.6 45.0 57.9 57.3 688 bp 1.0 66.1
rps16-trnK-UUU_p4 GGTGCTCAACCTACAAGAACT CGCACTTAAAAGCCGAGTAC 47.6 50.0 57.9 57.2 687 bp 1.0 65.7
rps16-trnK-UUU_p5 GGTGCTCAACCTACAAGAACT GTCGCACTTAAAAGCCGAGT 47.6 50.0 57.9 58.9 689 bp 1.0 65.1