Hotspot

trnQ-UUG-rps16

LSC · 75990-77535 · 1546 bp

Back to Indocalamus

Pi 0.00199
MarkerSeek score 34.20
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
1546
variable_sites
9
pi
0.00199
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC AAGTCGCACGTTGCTTTCTA 55.0 45.0 58.1 58.1 1604-1622 bp 1.0 80.0
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC TCATGTCCTTCAAGTCGCAC 55.0 50.0 58.1 58.2 1615-1633 bp 1.0 79.4
trnQ-UUG-rps16_p3 CTCGGAGGTTCGAATCCTTC AAGTCGCACGTTGCTTTCTA 55.0 45.0 57.8 58.1 1608-1626 bp 1.0 78.9
trnQ-UUG-rps16_p4 CTCGGAGGTTCGAATCCTTC TCATGTCCTTCAAGTCGCAC 55.0 50.0 57.8 58.2 1619-1637 bp 1.0 78.3
trnQ-UUG-rps16_p5 TAAAACCAGAAATGGGGCGT AAGTCGCACGTTGCTTTCTA 45.0 45.0 57.7 58.1 1664-1682 bp 1.0 78.1