Hotspot

psbM-trnD-GUC

LSC · 64555-65536 · 982 bp

Back to Indocalamus

Pi 0.00178
MarkerSeek score 44.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
982
variable_sites
7
pi
0.00178
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGCGAGAATATTGACTTCCA TTCAATTGGTCAGAGCACCG 40.0 50.0 54.5 58.5 1067-1075 bp 1.0 48.5
psbM-trnD-GUC_p2 TGCGAGAATATTGACTTCCA TAGGTATGCCATACACCCCG 40.0 55.0 54.5 58.7 1099-1107 bp 1.0 47.1
psbM-trnD-GUC_p3 ATGCGAGAATATTGACTTCCA TTCAATTGGTCAGAGCACCG 38.1 50.0 55.1 58.5 1068-1076 bp 1.0 45.5
psbM-trnD-GUC_p4 TGCGAGAATATTGACTTCCAT TTCAATTGGTCAGAGCACCG 38.1 50.0 55.1 58.5 1067-1075 bp 1.0 45.5
psbM-trnD-GUC_p5 TGCGAGAATATTGACTTCCA GTTCAATTGGTCAGAGCACC 40.0 50.0 54.5 57.0 1068-1076 bp 1.0 44.4