Hotspot

psbJ-petA

LSC · 19116-20099 · 984 bp

Back to Indocalamus

Pi 0.00322

MarkerSeek score 44.70

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 9
pi: 0.00322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TCCTCTTTGGCTGATAGGTACT	GGCCTTTTGTTCTTCTTCGC	45.5	50.0	58.0	57.9	1186 bp	1.0	64.2
psbJ-petA_p2	CCTCTTTGGCTGATAGGTACTG	GGCCTTTTGTTCTTCTTCGC	50.0	50.0	57.9	57.9	1185 bp	1.0	63.7
psbJ-petA_p3	TTCCTCTTTGGCTGATAGGT	GGCCTTTTGTTCTTCTTCGC	45.0	50.0	55.8	57.9	1187 bp	1.0	62.8
psbJ-petA_p4	ACGGTTCATATTCTGGATTGGG	GGCCTTTTGTTCTTCTTCGC	45.5	50.0	58.2	57.9	1121 bp	1.0	62.8
psbJ-petA_p5	CGGTTCATATTCTGGATTGGGT	GGCCTTTTGTTCTTCTTCGC	45.5	50.0	58.2	57.9	1120 bp	1.0	62.8