Hotspot

rps12-rpl20

LSC · 13126-13821 · 696 bp

Back to Indocalamus

Pi 0.000639

MarkerSeek score 44.80

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 2
pi: 0.000639
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	GAGGAACATGTGCTAGGGTG	ACGCATTTTTGTTCGTCGTC	55.0	45.0	58.0	58.0	773 bp	1.0	79.9
rps12-rpl20_p2	CGACATGCCAACGGTTAAAC	ACGCATTTTTGTTCGTCGTC	50.0	45.0	58.0	58.0	868 bp	1.0	79.7
rps12-rpl20_p3	GTCGAGGAACATGTGCTAGG	ACGCATTTTTGTTCGTCGTC	55.0	45.0	58.1	58.0	776 bp	1.0	79.5
rps12-rpl20_p4	GAGGAACATGTGCTAGGGTG	GTTTGAGGCAAATGAACGCA	55.0	45.0	58.0	57.9	788 bp	1.0	78.9
rps12-rpl20_p5	CGACATGCCAACGGTTAAAC	GTTTGAGGCAAATGAACGCA	50.0	45.0	58.0	57.9	883 bp	1.0	78.8