Hotspot

psbB-clpP

LSC · 11721-12215 · 495 bp

Back to Indocalamus

Pi 0.00135

MarkerSeek score 46.80

Primer available yes

Label psbB-clpP

Feature Metadata

label_name: psbB-clpP
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	TATGCACCGAAAGCAATCGA	ACCCAAGTCGCTTCTTCATC	45.0	50.0	58.0	57.9	625-627 bp	1.0	79.7
psbB-clpP_p2	TATGCACCGAAAGCAATCGA	TCAACCCAAGTCGCTTCTTC	45.0	50.0	58.0	58.1	628-630 bp	1.0	79.5
psbB-clpP_p3	TATGCACCGAAAGCAATCGA	CTTTTGGAACACCAATGGGC	45.0	50.0	58.0	57.8	585-587 bp	1.0	79.2
psbB-clpP_p4	TATGCACCGAAAGCAATCGA	TTTGGAACACCAATGGGCAT	45.0	45.0	58.0	58.3	583-585 bp	1.0	78.4
psbB-clpP_p5	TATGCACCGAAAGCAATCGA	AAGTCGCTTCTTCATCTCCG	45.0	50.0	58.0	57.7	621-623 bp	1.0	78.2