Hotspot

ndhF-rpl32

SSC · 117462-118260 · 799 bp

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Pi 0.0629

MarkerSeek score 81.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 799
variable_sites: 173
pi: 0.0629
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATGAAGGGGAAATTGGGCAG	AAAGGACTGTTGGCACTAGA	50.0	45.0	58.1	56.4	1791-2152 bp	0.782609	69.3
ndhF-rpl32_p2	TGTTGCCGTGGGAAAAAGTA	AAAGGACTGTTGGCACTAGA	45.0	45.0	57.9	56.4	1149-1510 bp	0.73913	68.2
ndhF-rpl32_p3	AGCAACCGAGCCACAAAATA	AAAGGACTGTTGGCACTAGA	45.0	45.0	58.1	56.4	1834-2128 bp	0.608696	62.6
ndhF-rpl32_p4	ATTTAGCAACCGAGCCACAA	AAAGGACTGTTGGCACTAGA	45.0	45.0	58.1	56.4	1838-2132 bp	0.608696	62.6
ndhF-rpl32_p5	TAGCAACCGAGCCACAAAAT	AAAGGACTGTTGGCACTAGA	45.0	45.0	58.1	56.4	1835-2129 bp	0.608696	62.6