Hotspot

rps3-rps19

LSC · 89052-89572 · 521 bp

Back to Indigofera

Pi 0.0403

MarkerSeek score 76.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 97
pi: 0.0403
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	204-637 bp	1.0	87.9
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	205-638 bp	1.0	87.8
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	CCGAGGACATGCGAAAAATG	50.0	50.0	58.4	57.8	203-636 bp	1.0	87.3
rps3-rps19_p4	AAGTCTGAAACCAAGCGGAT	TCCGAGGACATGCGAAAAAT	45.0	45.0	57.4	57.9	203-636 bp	1.0	86.6
rps3-rps19_p5	AGTCTGAAACCAAGCGGATT	TCCGAGGACATGCGAAAAAT	45.0	45.0	57.4	57.9	202-635 bp	1.0	86.6