Hotspot

petA-psbJ

LSC · 66753-67962 · 1210 bp

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Pi 0.0269

MarkerSeek score 86.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1210
variable_sites: 144
pi: 0.0269
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1298-1407 bp	1.0	84.6
petA-psbJ_p2	GATCCGTTACGTGTCCAAGG	TATTCCGGATTGGGCTCATC	55.0	50.0	58.4	57.1	1296-1405 bp	1.0	82.2
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	TCATATTCCGGATTGGGCTC	55.0	50.0	58.4	57.1	1299-1408 bp	1.0	82.2
petA-psbJ_p4	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCAT	55.0	47.6	58.4	57.9	1298-1407 bp	1.0	80.5
petA-psbJ_p5	GATCCGTTACGTGTCCAAGG	GTTCATATTCCGGATTGGGCT	55.0	47.6	58.4	58.1	1301-1410 bp	1.0	80.0