Hotspot

accD-psaI

LSC · 62997-63588 · 592 bp

Back to Indigofera

Pi 0.0612

MarkerSeek score 76.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 135
pi: 0.0612
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATACCCGATGGTTCACAAGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	683-1133 bp	1.0	91.5
accD-psaI_p2	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	738-1188 bp	0.956522	90.0
accD-psaI_p3	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	778-832 bp	0.086957	43.5
accD-psaI_p4	TTGATTCAATCGTACCGCGT	TTGTTTTTCGAGACCCGGTT	45.0	45.0	58.0	58.0	1075 bp	0.043478	41.4
accD-psaI_p5	TGCTTACATTGCATTTGCGG	TTGTTTTTCGAGACCCGGTT	45.0	45.0	58.0	58.0	1179 bp	0.043478	41.4