Hotspot

rps16-accD

LSC · 60730-61406 · 677 bp

Back to Indigofera

Pi 0.0393

MarkerSeek score 77.80

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 106
pi: 0.0393
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	CCGCAGAGAGGGAAGAGATA	TCTGGATGTTCCTGACACCT	55.0	50.0	58.0	58.0	935-1150 bp	1.0	91.7
rps16-accD_p2	GCCGTACGAGGAGAAAACTT	TCTGGATGTTCCTGACACCT	50.0	50.0	57.9	58.0	1041-1256 bp	1.0	91.2
rps16-accD_p3	GGCAAGAAAAGGGGGTAGAG	TCTGGATGTTCCTGACACCT	55.0	50.0	57.9	58.0	1272-1488 bp	0.913043	86.1
rps16-accD_p4	CAGGCAAGAAAAGGGGGTAG	TCTGGATGTTCCTGACACCT	55.0	50.0	58.2	58.0	1274-1490 bp	0.913043	85.9
rps16-accD_p5	AATTGATGTTCGATCCCGCA	TCTGGATGTTCCTGACACCT	45.0	50.0	58.0	58.0	1133-1141 bp	0.086957	43.6