Hotspot

trnQ-UUG-rps16

LSC · 59117-59611 · 495 bp

Back to Indigofera

Pi 0.0385
MarkerSeek score 79.10
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
495
variable_sites
77
pi
0.0385
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC ACGTTGCTTTTTGCCACATC 55.0 45.0 58.1 58.1 546-573 bp 1.0 90.2
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC GTTGCTTTTTGCCACATCGT 55.0 45.0 58.1 58.1 544-571 bp 1.0 90.2
trnQ-UUG-rps16_p3 GAGGTTCGAATCCTTCCGTC CACGTTGCTTTTTGCCACAT 55.0 45.0 58.1 58.4 547-574 bp 1.0 87.9
trnQ-UUG-rps16_p4 GAGGTTCGAATCCTTCCGTC TGCTTTTTGCCACATCGTTT 55.0 40.0 58.1 57.4 542-569 bp 1.0 86.6
trnQ-UUG-rps16_p5 GAGGTTCGAATCCTTCCGTC TTGCTTTTTGCCACATCGTT 55.0 40.0 58.1 57.4 543-570 bp 1.0 86.6