Hotspot

atpH-atpF

LSC · 51675-52266 · 592 bp

Back to Indigofera

Pi 0.0437

MarkerSeek score 95.40

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 108
pi: 0.0437
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 95.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGGCAGAGGGAAAAATACGG	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.6	57.7	750-871 bp	1.0	86.3
atpH-atpF_p2	GCAGAGGGAAAAATACGGGG	CCAGTAACCCAAGCAAAGGA	55.0	50.0	58.6	57.7	748-869 bp	1.0	84.8
atpH-atpF_p3	GGCAGAGGGAAAAATACGGG	CCAGTAACCCAAGCAAAGGA	55.0	50.0	58.6	57.7	749-870 bp	1.0	84.8
atpH-atpF_p4	CGGAGGCAGAGGGAAAAATA	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.3	57.7	753-874 bp	1.0	84.0
atpH-atpF_p5	CAGAGGGAAAAATACGGGGT	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.2	57.7	747-868 bp	1.0	83.3