Hotspot

ndhJ-trnF-GAA

LSC · 13270-14073 · 804 bp

Back to Indigofera

Pi 0.0299

MarkerSeek score 77.50

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 111
pi: 0.0299
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	GATGGCCTCTGCGTAAAGAT	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.8	58.1	876-950 bp	1.0	89.6
ndhJ-trnF-GAA_p2	ATAGGATGGCCTCTGCGTAA	CCTCGTGTCACCAGTTCAAA	50.0	50.0	58.3	58.1	880-954 bp	1.0	89.3
ndhJ-trnF-GAA_p3	ATGCCCGAAAGTTGGATAGG	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.7	58.1	895-969 bp	1.0	88.8
ndhJ-trnF-GAA_p4	AGTTGGATAGGATGGCCTCT	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.5	58.1	886-960 bp	1.0	87.6
ndhJ-trnF-GAA_p5	TAGGATGGCCTCTGCGTAAA	CCTCGTGTCACCAGTTCAAA	50.0	50.0	58.5	58.1	879-953 bp	1.0	87.5