Hotspot

trnK-UUU-rbcL

LSC · 4500-5891 · 1392 bp

Back to Indigofera

Pi 0.0494

MarkerSeek score 84.10

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1392
variable_sites: 279
pi: 0.0494
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	AGTCCTGAATTAGCTGCTGC	47.6	50.0	58.0	58.0	1401-1784 bp	1.0	83.6
trnK-UUU-rbcL_p2	AGCCGAGTACTCTACCATTGA	CCGTGAAGCTAGCAAATGGA	47.6	50.0	58.0	58.3	1420-1803 bp	1.0	81.8
trnK-UUU-rbcL_p3	AGCCGAGTACTCTACCATTGA	TCCGTGAAGCTAGCAAATGG	47.6	50.0	58.0	58.3	1421-1804 bp	1.0	81.8
trnK-UUU-rbcL_p4	GCCGAGTACTCTACCATTGAG	AGTCCTGAATTAGCTGCTGC	52.4	50.0	57.6	58.0	1400-1783 bp	1.0	80.9
trnK-UUU-rbcL_p5	AGCCGAGTACTCTACCATTGA	GCTGCTGCTTGTGAAGTATG	47.6	50.0	58.0	57.5	1389-1772 bp	1.0	79.6