Hotspot

rrn16-rps12

IRa · 142764-144680 · 1917 bp

Back to Incarvillea

Pi 0.0402

MarkerSeek score 85.10

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: IRa
length_bp: 1917
variable_sites: 72
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	CCGACTTGCATGTGTTAAGC	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1966-2026 bp	1.0	82.8
rrn16-rps12_p2	GCAGGTTCTTACGCGTTACT	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.3	57.7	2019-2079 bp	1.0	82.4
rrn16-rps12_p3	TTCTTACGCGTTACTCACCC	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	2014-2074 bp	1.0	81.9
rrn16-rps12_p4	CCAGGATCGAACTCTCCATG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.5	57.7	1923-1983 bp	1.0	80.7
rrn16-rps12_p5	CGTCCGACTTGCATGTGTTA	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.6	57.7	1969-2029 bp	1.0	80.2