Hotspot

rbcL-psaI

LSC · 59204-61895 · 2692 bp

Back to Incarvillea

Pi 0.42

MarkerSeek score 79.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 2692
variable_sites: 981
pi: 0.42
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGAAGGACGTGATCTTGCTG	AAAGAAGCCATTGCCATTGC	50.0	45.0	57.9	57.9	2932 bp	0.5	58.5
rbcL-psaI_p2	TGCCAAATGGAGTCCTGAAC	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.1	57.9	2884 bp	0.5	58.4
rbcL-psaI_p3	TTTGCGGCAGTGGATACTTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	58.1	57.9	2823 bp	0.5	58.4
rbcL-psaI_p4	TTTGCGGCAGTGGATACTTT	TTCGCCGGAAATCACATCTT	45.0	45.0	58.1	57.9	2966 bp	0.5	58.4
rbcL-psaI_p5	CCGCTTGTGAGGTATGGAAA	AAAGAAGCCATTGCCATTGC	50.0	45.0	57.9	57.9	2858 bp	0.5	58.3