Hotspot

trnT-UGU-trnL-UAA

LSC · 48604-49378 · 775 bp

Back to Incarvillea

Pi 0.0705

MarkerSeek score 85.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 52
pi: 0.0705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	819-836 bp	1.0	85.6
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	851-868 bp	1.0	84.4
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	813-830 bp	1.0	84.3
trnT-UGU-trnL-UAA_p4	AAAATAGGAAAAGGCCGGCT	CCGTAGCGTCTACCGATTTC	45.0	55.0	57.8	58.2	874-891 bp	1.0	84.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	851-868 bp	1.0	83.2