Hotspot

trnS-GGA-trnT-UGU

LSC · 47291-48530 · 1240 bp

Back to Incarvillea

Pi 0.0621

MarkerSeek score 81.30

Primer available yes

Label trnS-GGA-trnT-UGU

Feature Metadata

label_name: trnS-GGA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1240
variable_sites: 76
pi: 0.0621
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnT-UGU_p1	GCTTCTTCCCTTAGGCTGTC	GTTCTGCACGATTGGCTCTA	55.0	50.0	58.0	58.0	2903 bp	0.5	59.7
trnS-GGA-trnT-UGU_p2	AGGTGTAGTGCTTCTTCCCT	GTTCTGCACGATTGGCTCTA	50.0	50.0	58.0	58.0	2912 bp	0.5	59.7
trnS-GGA-trnT-UGU_p3	ACTTCACCTTCAACAAGCGT	GTTCTGCACGATTGGCTCTA	45.0	50.0	58.0	58.0	2736 bp	0.5	59.6
trnS-GGA-trnT-UGU_p4	TTCTTCCCTTAGGCTGTCCA	GTTCTGCACGATTGGCTCTA	50.0	50.0	58.0	58.0	2901 bp	0.5	59.6
trnS-GGA-trnT-UGU_p5	AGGCTTTTGTTTACCGAGGG	GGAACCGCTAACCAAAAAGC	50.0	50.0	58.1	57.9	2754 bp	0.5	58.8