Hotspot

atpH-atpI

LSC · 15536-16762 · 1227 bp

Back to Incarvillea

Pi 0.471

MarkerSeek score 78.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 450
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGTCCCTTGACCAATT	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.1	58.0	1529-1652 bp	1.0	91.0
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.1	58.0	1465-1588 bp	1.0	90.9
atpH-atpI_p3	CAGCAGTCCCTTGACCAATT	CAAACTATTCCGACTGGGGG	50.0	55.0	58.1	58.0	1882 bp	0.5	59.0
atpH-atpI_p4	CAGCAGTCCCTTGACCAATT	GAGAAGAATACGGCCCTTGG	50.0	55.0	58.1	58.0	1803 bp	0.5	59.0
atpH-atpI_p5	CAGCAGTCCCTTGACCAATT	GGAGAAGAATACGGCCCTTG	50.0	55.0	58.1	58.0	1804 bp	0.5	59.0