Hotspot

matK-trnQ-UUG

LSC · 4823-8940 · 4118 bp

Back to Incarvillea

Pi 0.141

MarkerSeek score 78.70

Primer available yes

Label matK-trnQ-UUG

Feature Metadata

label_name: matK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 4118
variable_sites: 544
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnQ-UUG_p1	AGACCATCCAGTAGCCTGTT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.0	57.9	766 bp	0.5	59.0
matK-trnQ-UUG_p2	TCTCGGGGCCTTCTTTTCTA	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	851 bp	0.5	58.9
matK-trnQ-UUG_p3	AGACCATCCAGTAGCCTGTT	TCTGGGGTCCCCTGATAATC	50.0	55.0	58.0	57.9	393 bp	0.5	58.7
matK-trnQ-UUG_p4	AGACCATCCAGTAGCCTGTT	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.0	57.9	754 bp	0.5	58.7
matK-trnQ-UUG_p5	TCTCGGGGCCTTCTTTTCTA	TCTGGGGTCCCCTGATAATC	50.0	55.0	58.1	57.9	478 bp	0.5	58.6