Hotspot

ycf1

SSC · 122360-127960 · 5601 bp

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Pi 0.0574

MarkerSeek score 78.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 2685
pi: 0.0574
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAAGTGGAATGCTATCCCA	GCGCCCAAAGGAAGAAAATC	50.0	50.0	57.9	58.0	277-304 bp	0.253731	61.7
ycf1_p2	AGTTCAGTCTCCATTCCCCA	GCGCCCAAAGGAAGAAAATC	50.0	50.0	58.0	58.0	646-673 bp	0.208955	60.0
ycf1_p3	AGTTCAGTCTCCATTCCCCA	GAAGAAGTGCAAGTGGGACA	50.0	50.0	58.0	58.0	836-863 bp	0.119403	54.0
ycf1_p4	TCTGCTATTTCTATCCGCGC	GCGCCCAAAGGAAGAAAATC	50.0	50.0	57.9	58.0	280 bp	0.089552	44.2
ycf1_p5	CTGCTATTTCTATCCGCGCT	GCGCCCAAAGGAAGAAAATC	50.0	50.0	57.9	58.0	279 bp	0.089552	44.1