Hotspot

ndhF-rpl32

SSC · 111281-112093 · 813 bp

Back to Impatiens

Pi 0.0942

MarkerSeek score 77.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 813
variable_sites: 443
pi: 0.0942
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAGGCCGTGTAAACCAAAAC	AGGCTTTTAACGCTGTCCAA	50.0	45.0	57.9	58.0	952-1418 bp	0.925373	87.8
ndhF-rpl32_p2	CAGGCCGTGTAAACCAAAAC	TTCACCATAGCGGCTTTCTC	50.0	50.0	57.9	58.0	1964-2040 bp	0.208955	59.1
ndhF-rpl32_p3	GGCAAATAATGGAACGGCAC	AGGCTTTTAACGCTGTCCAA	50.0	45.0	58.1	58.0	1597-1612 bp	0.074627	44.4
ndhF-rpl32_p4	GGCAAATAATGGAACGGCAC	AAGGCTTTTAACGCTGTCCA	50.0	45.0	58.1	58.0	1598-1613 bp	0.074627	44.3
ndhF-rpl32_p5	GGCAAATAATGGAACGGCAC	TTCACCATAGCGGCTTTCTC	50.0	50.0	58.1	58.0	2219-2234 bp	0.059701	43.4