Hotspot

ndhC-trnV-UAC

LSC · 50402-50844 · 443 bp

Back to Impatiens

Pi 0.063

MarkerSeek score 80.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 179
pi: 0.063
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGTTCAGGCATTTGCTCAT	TGGTAGTGCTCCTGGATCAA	45.0	50.0	58.1	58.0	1167-1490 bp	0.955224	89.2
ndhC-trnV-UAC_p2	TGGTTCAGGCATTTGCTCAT	TTGGTAGTGCTCCTGGATCA	45.0	50.0	58.1	58.0	1168-1491 bp	0.955224	89.2
ndhC-trnV-UAC_p3	TGGTTCAGGCATTTGCTCAT	CCGAGAAAGTCTACGGTTCG	45.0	55.0	58.1	58.1	967-1283 bp	0.940299	88.2
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	CCGATTCAGGTGCCCATTTA	45.0	50.0	58.1	57.9	1438-1760 bp	0.910448	87.4
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	TGGTAGTGCTCCTGGATCAA	50.0	50.0	57.9	58.0	560-881 bp	0.835821	83.9