Hotspot

rpoB-trnC-GCA

LSC · 26012-27099 · 1088 bp

Back to Impatiens

Pi 0.0461

MarkerSeek score 80.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 335
pi: 0.0461
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTTGTTCTTGCATGTCCCGA	GCAGTGACATCGCACAAAAA	45.0	45.0	58.0	57.9	1533-2146 bp	0.880597	86.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	GCAGTGACATCGCACAAAAA	50.0	45.0	58.0	57.9	1753-2366 bp	0.880597	86.1
rpoB-trnC-GCA_p3	TTTGTTCTTGCATGTCCCGA	CTTTTGGAAACACGCAGACC	45.0	50.0	58.0	57.9	1404-1656 bp	0.880597	86.1
rpoB-trnC-GCA_p4	GAAAATACCGGATCCCCACC	CTTTTGGAAACACGCAGACC	55.0	50.0	58.0	57.9	1831-2083 bp	0.835821	84.3
rpoB-trnC-GCA_p5	GAAAATACCGGATCCCCACC	GCAGTGACATCGCACAAAAA	55.0	45.0	58.0	57.9	2218-2573 bp	0.820896	83.7