Hotspot

trnK-UUU-rps16

LSC · 4261-4926 · 666 bp

Back to Impatiens

Pi 0.051

MarkerSeek score 78.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 246
pi: 0.051
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGTTCCGCAACTGGTACTAA	AAAAGGGTATGTTGCTGCCA	50.0	45.0	57.9	58.0	1614-1724 bp	0.925373	88.4
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1343-1455 bp	0.925373	88.3
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1347-1459 bp	0.850746	85.8
trnK-UUU-rps16_p4	CGAATCCTCGCTTCATCCAA	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1300-1412 bp	0.820896	84.3
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TAACTGCCCCAGAATGAGGA	55.0	50.0	58.0	58.0	1225-1339 bp	0.701493	79.7