Hotspot

rpoC2

LSC · 17318-21487 · 4170 bp

Back to Illigera

Pi 0.00687

MarkerSeek score 44.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4170
variable_sites: 43
pi: 0.00687
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGTATGCACTTCTTCGGGAA	ACCCATAAATGGTATTTTACGT	50.0	31.8	57.6	53.5	220 bp	1.0	42.2
rpoC2_p2	AAGGTATGCACTTCTTCGGG	ACCCATAAATGGTATTTTACGT	50.0	31.8	57.6	53.5	222 bp	1.0	42.2
rpoC2_p3	AGGTATGCACTTCTTCGGGA	ACCCATAAATGGTATTTTACGT	50.0	31.8	58.4	53.5	221 bp	1.0	42.2
rpoC2_p4	TGCACTTCTTCGGGAATGAA	ACCCATAAATGGTATTTTACGT	45.0	31.8	57.4	53.5	216 bp	1.0	42.2
rpoC2_p5	GGTATGCACTTCTTCGGGAA	TCTTGTATCTTGATTGAAATACCCA	50.0	32.0	57.6	56.3	240 bp	1.0	42.0