Hotspot

trnN-GUU-trnR-ACG

IRa · 131665-132235 · 571 bp

Back to Illigera

Pi 0.0108

MarkerSeek score 67.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 571
variable_sites: 9
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	676-685 bp	1.0	79.9
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	663-672 bp	1.0	79.9
trnN-GUU-trnR-ACG_p3	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	664-673 bp	1.0	79.5
trnN-GUU-trnR-ACG_p4	ATCCCCCGTTATTCCTCAGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	717-726 bp	1.0	79.4
trnN-GUU-trnR-ACG_p5	CGGGAGTTGCACTCTATGAG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	742-751 bp	1.0	79.1