Hotspot

rpl2-rpl23

IRb · 86892-87118 · 227 bp

Back to Illigera

Pi 0.0166

MarkerSeek score 55.20

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 227
variable_sites: 5
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TTTCGCTTGACTGTCTACGG	CGAATCAGGATCTAATAGGACGG	50.0	47.8	57.9	58.0	311-325 bp	1.0	60.7
rpl2-rpl23_p2	GACTGTCTACGGCTCCATTG	CGAATCAGGATCTAATAGGACGG	55.0	47.8	58.1	58.0	303-317 bp	1.0	60.6
rpl2-rpl23_p3	TCGTGGATTGGATTTCGCTT	CGAATCAGGATCTAATAGGACGG	45.0	47.8	57.9	58.0	323-337 bp	1.0	60.1
rpl2-rpl23_p4	TTCGTGGATTGGATTTCGCT	CGAATCAGGATCTAATAGGACGG	45.0	47.8	57.9	58.0	324-338 bp	1.0	60.1
rpl2-rpl23_p5	CGTGGATTGGATTTCGCTTG	CGAATCAGGATCTAATAGGACGG	50.0	47.8	57.8	58.0	322-336 bp	1.0	59.6