Hotspot

petA-psbJ

LSC · 64232-65397 · 1166 bp

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Pi 0.0189

MarkerSeek score 73.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 32
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGTT	TCCGGATTAGGATCATCCCT	50.0	50.0	57.8	57.0	1275-1308 bp	1.0	74.6
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	TCCGGATTAGGATCATCCCT	55.0	50.0	58.3	57.0	1277-1310 bp	1.0	74.0
petA-psbJ_p3	CCCATTACGTGTCCAAGGTT	TTCCGGATTAGGATCATCCCT	50.0	47.6	57.8	57.7	1276-1309 bp	1.0	72.6
petA-psbJ_p4	CCCATTACGTGTCCAAGGTT	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	1326-1359 bp	1.0	72.4
petA-psbJ_p5	CCCATTACGTGTCCAAGGTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1325-1358 bp	1.0	72.4