Hotspot

ycf3-trnS-GGA

LSC · 45711-46533 · 823 bp

Back to Illigera

Pi 0.0146

MarkerSeek score 68.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 17
pi: 0.0146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	791-1012 bp	1.0	71.6
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	792-1013 bp	1.0	71.6
ycf3-trnS-GGA_p3	TCTCCTGAAGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.2	58.2	790-1011 bp	1.0	69.7
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.2	58.2	792-1013 bp	1.0	69.6
ycf3-trnS-GGA_p5	TTTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.2	58.2	793-1014 bp	1.0	69.6