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psbZ-trnG-GCC

LSC · 37455-37716 · 262 bp

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Pi 0.092

MarkerSeek score 89.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 36
pi: 0.092
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGCTTCTTCTGATGGTTGGT	AATCGAACCCGCATCTTCTC	45.0	50.0	57.0	58.1	428-430 bp	1.0	79.5
psbZ-trnG-GCC_p2	TGCTTCTTCTGATGGTTGGT	GAATCGAACCCGCATCTTCT	45.0	50.0	57.0	58.1	429-431 bp	1.0	79.5
psbZ-trnG-GCC_p3	GCTTCTTCTGATGGTTGGTCA	AATCGAACCCGCATCTTCTC	47.6	50.0	58.2	58.1	427-429 bp	1.0	77.5
psbZ-trnG-GCC_p4	GCTTCTTCTGATGGTTGGTCA	GAATCGAACCCGCATCTTCT	47.6	50.0	58.2	58.1	428-430 bp	1.0	77.4
psbZ-trnG-GCC_p5	TGCTTCTTCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	47.6	50.0	58.2	58.1	428-430 bp	1.0	77.4