Hotspot

psbM-trnD-GUC

LSC · 30441-31475 · 1035 bp

Back to Illigera

Pi 0.0161

MarkerSeek score 67.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 25
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	TGCTGTACGCCTCATTTCTC	42.9	50.0	58.2	58.0	1177-1183 bp	1.0	71.9
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	TGCTGTACGCCTCATTTCTC	45.0	50.0	56.7	58.0	1176-1182 bp	1.0	71.7
psbM-trnD-GUC_p3	AGAATGAACAACGCAGTAGCA	GTACGCCTCATTTCTCTGGG	42.9	55.0	58.2	58.1	1173-1179 bp	1.0	71.5
psbM-trnD-GUC_p4	GAATGAACAACGCAGTAGCA	GTACGCCTCATTTCTCTGGG	45.0	55.0	56.7	58.1	1172-1178 bp	1.0	71.4
psbM-trnD-GUC_p5	AGAATGAACAACGCAGTAGCA	TACGCCTCATTTCTCTGGGA	42.9	50.0	58.2	58.1	1172-1178 bp	1.0	70.9