Hotspot

atpH-atpI

LSC · 14349-15436 · 1088 bp

Back to Illigera

Pi 0.0203

MarkerSeek score 71.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 29
pi: 0.0203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	975-1152 bp	1.0	85.3
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	991-1168 bp	1.0	84.4
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	976-1153 bp	1.0	84.1
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.8	57.9	990-1167 bp	1.0	83.9
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.3	57.9	985-1162 bp	1.0	83.5