Hotspot

atpF-atpH

LSC · 13660-14102 · 443 bp

Back to Illigera

Pi 0.0108

MarkerSeek score 64.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 7
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTGGCCAATGATCCAAGGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.1	58.0	532-543 bp	1.0	81.3
atpF-atpH_p2	AAATCCGAAACTCCCTGCAG	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.2	58.0	552-563 bp	1.0	81.0
atpF-atpH_p3	GTGGCCAATGATCCAAGGAA	CAGAGGGCAAAATACGAGGT	50.0	50.0	58.1	57.6	591-602 bp	1.0	78.1
atpF-atpH_p4	GCCAATGATCCAAGGAAACG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.4	58.0	529-540 bp	1.0	78.0
atpF-atpH_p5	AAATCCGAAACTCCCTGCAG	CAGAGGGCAAAATACGAGGT	50.0	50.0	58.2	57.6	611-622 bp	1.0	77.9