Hotspot

ycf2

Genome · 89638-96510 · 6873 bp

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Pi 0.00298

MarkerSeek score 43.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6873
variable_sites: 86
pi: 0.00298
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAA	45.0	45.0	56.2	57.2	240-243 bp	1.0	66.8
ycf2_p2	AACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAA	42.9	45.0	57.0	57.2	241-244 bp	1.0	64.9
ycf2_p3	TCAACGGAGGAACAATATCACC	AGAACCAGAAGCGATGTGAA	45.5	45.0	58.1	57.2	243-246 bp	1.0	63.7
ycf2_p4	ACGGAGGAACAATATCACCAT	AGAACCAGAAGCGATGTGAA	42.9	45.0	56.8	57.2	240-243 bp	1.0	63.2
ycf2_p5	ACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAAT	45.0	42.9	56.2	57.7	240-243 bp	1.0	62.8