Hotspot

rpl32-trnL-UAG

Genome · 111199-112751 · 1553 bp

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Pi 0.00777

MarkerSeek score 63.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1553
variable_sites: 47
pi: 0.00777
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGACCGCAGTAAAAGCTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1594-1738 bp	1.0	85.1
rpl32-trnL-UAG_p2	TTGGACCGCAGTAAAAGCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1595-1739 bp	1.0	85.1
rpl32-trnL-UAG_p3	TGGACCGCAGTAAAAGCTTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	1587-1731 bp	1.0	84.6
rpl32-trnL-UAG_p4	TTGGACCGCAGTAAAAGCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	1588-1732 bp	1.0	84.6
rpl32-trnL-UAG_p5	TGGACCGCAGTAAAAGCTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.0	57.8	1592-1736 bp	1.0	84.5