Hotspot

ndhF-rpl32

Genome · 109757-111039 · 1283 bp

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Pi 0.00843

MarkerSeek score 60.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1283
variable_sites: 40
pi: 0.00843
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACAAGCAGGAGTCCCAATC	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.8	57.7	986-1398 bp	1.0	82.3
ndhF-rpl32_p2	GAACAAGCAGGAGTCCCAAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.8	57.7	987-1399 bp	1.0	82.3
ndhF-rpl32_p3	AAGCAGGAGTCCCAATCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.7	57.7	983-1395 bp	1.0	81.8
ndhF-rpl32_p4	AGCAGGAGTCCCAATCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.5	57.7	982-1394 bp	1.0	79.9
ndhF-rpl32_p5	CTTTTTGTTGCCGTCGGAAC	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.5	57.7	1003-1415 bp	1.0	79.7