Hotspot

trnL-CAA-trnV-GAC

Genome · 96642-98199 · 1558 bp

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Pi 0.00361
MarkerSeek score 57.60
Primer available yes
Label trnL-CAA-trnV-GAC

Feature Metadata

label_name
trnL-CAA-trnV-GAC
feature_type
igs
region
Genome
length_bp
1558
variable_sites
20
pi
0.00361
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnV-GAC_p1 GTCTCGCGTGTCTACCATTT CGGGGGAGCAAGTCAAAATA 50.0 50.0 58.0 57.9 1675-1819 bp 1.0 83.9
trnL-CAA-trnV-GAC_p2 CTCGCGTGTCTACCATTTCA CGGGGGAGCAAGTCAAAATA 50.0 50.0 58.0 57.9 1673-1817 bp 1.0 83.9
trnL-CAA-trnV-GAC_p3 GTCTCGCGTGTCTACCATTT GATGACTTCCACCACGTCAA 50.0 50.0 58.0 57.8 1614-1758 bp 1.0 83.6
trnL-CAA-trnV-GAC_p4 CTCGCGTGTCTACCATTTCA GATGACTTCCACCACGTCAA 50.0 50.0 58.0 57.8 1612-1756 bp 1.0 83.6
trnL-CAA-trnV-GAC_p5 GTCTCGCGTGTCTACCATTT ATGACTTCCACCACGTCAAG 50.0 50.0 58.0 57.8 1613-1757 bp 1.0 83.5