Hotspot

petA-psbJ

Genome · 66050-67180 · 1131 bp

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Pi 0.0134

MarkerSeek score 74.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1131
variable_sites: 39
pi: 0.0134
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTACGTGTCCAAGGGCTTC	GGCTCATATTCTGGGTTGGG	50.0	55.0	57.9	58.0	1252-1307 bp	1.0	85.5
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	GGCTCATATTCTGGGTTGGG	50.0	55.0	58.2	58.0	1170-1225 bp	1.0	85.1
petA-psbJ_p3	CATTACGTGTCCAAGGGCTT	GGCTCATATTCTGGGTTGGG	50.0	55.0	58.2	58.0	1253-1308 bp	1.0	84.9
petA-psbJ_p4	ATTACGTGTCCAAGGGCTTC	TGATCGGTTCAATGGGCATT	50.0	45.0	57.9	57.9	1281-1336 bp	1.0	84.5
petA-psbJ_p5	GTCCAAGGGCTTCTGTTCTT	GGCTCATATTCTGGGTTGGG	50.0	55.0	57.7	58.0	1245-1300 bp	1.0	84.2